In the initial FST pilot study by Chawla et al., 2013, the 2-h urine output after FST was significantly better than each urinary biomarker tested (IGFBP7/TIMP2 and urinary NGAL) in predicting progression to Stage 3 AKI (p < 0.05) [41]. The gene discussed is LCN2; the disease is acute kidney injury.