PTEN and neoplasm: The results showed that mean levels of the tumor markers (CEA and CA15.3) were significantly increased in blood samples of breast cancer patients as compared to the benign and control groups and the mean level of PTEN methylation increased significantly in BC patients, these findings proved that the frequency of PTEN methylation was significantly higher in breast cancer patients than in corresponding benign cases and control group (p < 0.001) which proposes that PTEN methylation might have a significant role in breast cancer developing.