To determine the extent to which EPSTI1 expression was sufficient to restore CRC cell viability in the absence of KSR1, we grew cells under anchorage-independent conditions either on Poly-(HEMA) (Figure 7C) or on soft agar (Figure 7D) following forced expression of EPSTI1 in HCT116, HCT15, and SW480 cells lacking KSR1. Here, KSR1 is linked to colorectal carcinoma.