Moreover, similar results were found in 3 HCC cell lines, HepG2 (Figure 4C and Supplementary Figure S5C), SMMC7721 (Figure 4D and Supplementary Figure S5D), and HepG2.2.15 (Supplementary Figure S5E), thus validating that CUL4B promotes the accumulation of HBx protein. This evidence concerns the gene CUL4B and hepatocellular carcinoma.