In the present study, we have elucidated that S100A7 can determine the fate of TLR4 in breast cancer cells, and the exogenous treatment of LPS/S100A7 or downregulation of TLR4 increases the expression of RAGE, thus confirming our previous report revealing an essential role of S100A7‐RAGE signaling in the enhancement of breast cancer growth and metastasis, especially in triple‐negative breast cancer [21]. This evidence concerns the gene TLR4 and breast cancer.