In 2019, Fu et al. (14) primarily performed scRNA-seq analysis in streptozotocin-induced diabetic endothelial nitric oxide synthase (eNOS)-deficient and control eNOS-deficient mice and revealed increased infiltrating macrophages in glomeruli, dynamic alterations in the pattern of expressed genes in glomerular endothelial cells and mesangial cells of DKD and control mice, and variable responses of individual cells. Here, NOS3 is linked to diabetic kidney disease.