TMPRSS2 and hepatocellular carcinoma: As scRNA-seq analysis indicated a higher number of ACE2+/TMPRSS2+/TROP2+ co-expressing cells in cirrhotic liver, we used the RNA-FISH approach to validate these results in formalin-fixed paraffin-embedded (FFPE) tissues from the fatty (cirrhotic) liver, tumor, and adjacent normal sectors of hepatocellular carcinoma (Figures 4A–F).