CD163 and neoplasm: While no significant changes in CD206 or CD163 expression were observed when the A549 cell line was co-cultured with lung fibroblasts and THP-1 monocytes, tumor cell-derived PKH26 and fibroblast-derived PKH67 were within THP-1 cells expressing CD163 and CD206, suggesting tumor and fibroblast education of monocytes in this model as well (Supplemental Figure 5).