TP53 and lung adenocarcinoma: With this system, we generated two 3D models of lung adenocarcinoma, one using the A549 cell line, a KRAS mutant and p53 wild-type NSCLC cell line that resembles alveolar type II cells, and the other using the H358 cell line, which is a KRAS mutant, p53 null NSCLC cell line (26–29).