With respect to principles guiding p53 targeted therapies, our analysis corroborated previous Ad-p53 monotherapy clinical trials results that identified p53 biomarkers predictive of therapeutic efficacy defined by wild type p53 gene sequence, less than 20% p53 positive tumor cells by immunohistochemistry (IHC), or p53 gene mutations that will not inhibit normal p53 function such as gene deletions, truncations, or frame-shift mutations that have non-functional p53 tetramerization domains (7). Here, TP53 is linked to neoplasm.