Prior to western blotting, TLR4-overexpressing monocytes were pretreated with either dasatinib (an Src inhibitor), filipin (a strong caveolae-mediated endocytosis inhibitor), or TAK242 (a TLR4 inhibitor) and subjected to 30 min of E. coli infection. The gene discussed is SRC; the disease is escherichia coli infection.