TLR4 and neoplasm: Blockage of the Bgn-TLR2 or Bgn-TLR4 interaction in E0771 cells by neutralizing antibodies significantly decreased Tnf mRNA expression in E0771 cells stimulated with recombinant biglycan (Fig. 3k, l), suggesting that biglycan regulates TNF-ɑ expression in tumor cells through binding to TLR2 and TLR4.