To validate our atypical in vitro observation into an in vivo system, we picked three different colon cancer cell lines having different CXCR4 expression patterns, such as HT-29, has robust CXCR4 surface expression and other two DLD-1 and HCT-116 having only intracellular CXCR4 expression. The gene discussed is CXCR4; the disease is malignant colon neoplasm.