To validate our atypical in vitro observation into an in vivo system, we picked three different colon cancer cell lines having different CXCR4 expression patterns, such as HT-29, has robust CXCR4 surface expression and other two DLD-1 and HCT-116 having only intracellular CXCR4 expression. Here, CXCR4 is linked to colonic neoplasm.