A detailed analysis of PD-L1 expression in all 90 tissues specimen revealed significant overexpression of PD-L1 in glioma compared with healthy brain tissue: There was a statistically significant overexpression in glioma compared to cortex (p < 0.01, Fig. 2a) and white matter (p < 0.01, Fig. 2a). Here, CD274 is linked to central nervous system cancer.