Subsequently, we detected the expression levels of downstream NF-κB signaling-target genes related to tumor migration and invasion (urokinase-type plasminogen activator receptor [uPAR], intracellular adhesion molecule 1 [ICAM1], vascular cell adhesion molecule 1 [VCAM1], and matrix metalloproteinase 9 [MMP9]) by quantitative real-time polymerase chain reaction (qRT-PCR) and WB (Fig. 3b, c) analysis; their expression levels was significantly higher in ROC1-OE group than that in control group, while, ROC1 knockdown showed the opposite effects. Here, RBX1 is linked to neoplasm.