We found a strong relationship between rounded nestin+ cells and PpIX+ fluorescence at the edge of IDH1mut lower-grade gliomas (Figure 4C; slope = 0.003 ± 0.001, R2 = 0.85, P = .003, n = 7 IDH1mut gliomas), but no correlation with elongated nestin+ cells (Figure 4D; slope = 0.23 ± 0.03, R2 = 0.29, P = .22, n = 7 IDH1mut gliomas). Here, NES is linked to glioma.