To explore whether miR-182-5p participates in the regulation of liver cancer cell function by targeting RCAN1, cell lines with overexpressed RCAN1 (miR − NC + oe − RCAN1) or cell lines with simultaneously overexpressed miR-182-5p and RCAN1 (miR − mimic + oe − RCAN1) were constructed. The gene discussed is RCAN1; the disease is liver cancer.