In this study, expression of pyruvate carboxykinase (Pklr), pyruvate carboxylase (Pc), glycogen phosphorylase (Pyg1), and Phosphoglucomutase-1 (Pgm1) were significantly down-regulated in hypothyroidism rats, which indicated that the glycolysis pathway was blocked. The gene discussed is PKLR; the disease is hypothyroidism.