Recently, researchers have significantly prolonged the reproductive age of mice by short-term injection of rapamycin69; as PTEN-PI3K-AKT pathway controls follicle activation, doctors have activated the follicles in vitro by adding the PTEN (phosphatase and tensin homolog) inhibitor bpV, which successfully allowed women who have premature ovarian failure gave birth to healthy children70,71. This evidence concerns the gene PTEN and primary ovarian failure.