Treatment with TM4SF1 siRNA-2 led to a significant increase in the percentages of SA-β-gal- and SAHF-positive cells (Fig. 5A, B), as well as the protein level of p21 in B7-H3-overexpressing CRC cells treated with low-dose DOX (Fig. 5C). Here, TM4SF1 is linked to colorectal carcinoma.