As expected, our Western blot assay indicated that perifosine, an AKT inhibitor, markedly reduced the phosphorylation levels of AKT and the expression of TM4SF1 in low-dose DOX-induced senescent B7-H3-overexpressing CRC cells, providing evidence that B7-H3 modulated TM4SF1 expression in senescent CRC cells via the AKT signaling pathway. Here, TM4SF1 is linked to colorectal carcinoma.