For the USFLM group, the forest plot showed that there were no statistical differences in the patients with tumor size≤5 cm (HR 0.635; 95% CI 0.363–1.110) and negative CEA(HR 0.353; 95% CI 0.113–1.103) subgroups for OS when the efficacy of PTR to non-PTR was compared; there were no statistical differences in the patients with tumor size≤5 cm (HR 0.627; 95% CI 0.351–1.118) and negative CEA (HR 0.346; 95% CI 0.109–1.095) subgroups for CSS. This evidence concerns the gene CEACAM5 and neoplasm.