By comparing the proliferation activity, apoptosis rate, cell cycle ratio, migration and invasion ability of different groups of cells, and Wnt3a and P-GSK3β protein levels, the molecular mechanism of the 73HOXC-AS1 overexpression plasmid-activated Wntβ-catenin classic signaling pathway and LV-eIF4AIII-RNAi (44682-1)-increased eIF4AIII expression on gastric cancer BGC823 cells was comprehensively evaluated. The gene discussed is EIF4A3; the disease is gastric cancer.