In another longitudinal follow-up study of control subjects and patients of preclinical AD, MCI, and advanced AD, increased NOX activity was seen in the temporal cortex of MCI patients but not in those of preclinical AD or advanced AD subjects; immunohistochemical and immunoblotting analyses showed increased levels of gp91phox and p47phox in the MCI group [134]. The gene discussed is NCF1; the disease is Alzheimer disease.