Next, the expression levels of glycolysis-related genes were verified by real-time PCR at day 1 and 3 post-infection, indicating an obvious increase of key glycolytic enzymes and proteins in MEE (Figure 1B) and inflammatory cells of MELF (Figure 1C), including hk3, pfkfb3, pyruvate kinase M2 (pkm2), lactate dehydrogenase a (ldha), glucose uptake transporter 1 (glut1) and lactate secretion transporter 4 (mct4). This evidence concerns the gene PFKFB3 and infection.