As shown in Figure 5E, immunohistochemical quantitative analysis showed that the expression levels of cleaved-caspase 3, LC3B, and P62 in the FRA group were higher than those in the control group, which indicated that FRA also induced autophagy and apoptosis of osteosarcoma in vivo. In addition, as shown in Figure 5F, the vital organs of mice (including the heart, liver, spleen, lungs and kidneys) did not show significant toxicity throughout the experimental. The gene discussed is CASP3; the disease is osteosarcoma.