The gain of invasive capability was also associated with an increased expression of EMT genes (e.g., CDH2, FN1, VIM, SNAI1), as revealed by both RT-qPCR and WB analyses of bulk tumors (Fig. 3i, j; Supplementary Fig. 4h), Bdmc+/+ and Bdmc−/− cells (Supplementary Fig. 4i) and siAMBRA1 human melanoma SK-Mel-5 (Fig. 3k, l and Supplementary Fig. 4j), MeWo and SK-Mel-2 cells (Supplementary Fig. 4k–m). This evidence concerns the gene VIM and melanoma.