AMBRA1 and melanoma: Therefore, to assess whether distant organs were more efficiently colonized by Ambra1-deficient melanoma cells, we took advantage of a syngeneic mouse model, i.e., C57Bl/6 mice injected with freshly dissociated cells from BPA+/+ and BPA−/− tumors through the tail vein (sBPA+/+ and sBPA−/−, respectively) (Fig. 4e).