Finally, we sought to assess whether the expression levels of AMBRA1 could differently affect the sensitivity of human melanoma cell lines to FAKi. To this aim, we treated a panel of 15 melanoma cell lines with increasing doses of FAKi for 24 h (Supplementary Fig. 8a) and revealed a striking correlation between AMBRA1 protein expression levels and sensitivity to FAKi (Fig. 6j). Here, AMBRA1 is linked to melanoma.