AMBRA1 and melanoma: Notably, gelatin invadopodia assay showed that human SK-Mel-5 melanoma cells silenced for AMBRA1 (siAMBRA1) were characterized by a higher ability to degrade gelatin matrix, when compared to control (siScr) cells (Fig. 3f), thus highlighting possible implications of AMBRA1 deficiency in increased invasive and migratory capacity of melanoma cells.