In order to understand whether or not FAK1 played a role in the invasive and migratory ability observed upon AMBRA1 deficiency (Fig. 3g, h), we performed wound healing and transwell migration assays in siAMBRA1 melanoma cells concomitantly downregulating FAK1 (siAMBRA1:siFAK1 double knocked down) (Fig. 5h and Supplementary Fig. 7a). Here, PTK2 is linked to melanoma.