AMBRA1 and melanoma: To test whether the loss of Ambra1 was sufficient to lead to melanoma formation in BrafV600E-mutated melanocytes18, Tyr::CreERT2/+;BrafV600E/+;Ambra1+/+ (BA+/+) and Tyr::CreERT2/+;BrafV600E/+;Ambra1−/− (BA−/−) mice were treated with 4-OHT on the dorsal skin at postnatal days 1, 3, and 5 (Supplementary Fig. 1b).