In contrast to the transformed epithelial cell line HEK293, which sheds abundant EVs, EVs from HMC-1.1 (expressing the V560G-KIT variant) and particularly HMC-1.2 (expressing V560G-KIT and D816V-KIT, the most common variant associated with SM), produced substantial amounts of miR-21, miR-23-a, miR-25, miR-30a, and miR-93 (Fig. 6a). Here, KIT is linked to systemic mastocytosis.