The findings of this trial revealed that the supplementation with 3 g/day l-CIT for eight weeks significantly increased the serum levels of CIT and reduced fasting blood glucose concentrations, HbA1c without any significant effects on QUICKI and HOMA-B, serum IL-6, MCP-1, and TLR-4 concentrations compared to the placebo in the patients with T2D. This evidence concerns the gene TLR4 and type 2 diabetes mellitus.