In any case, given that the addition of IL12 to iso1Au/TNF did not impair its synergism with chemotherapy in the WEHI fibrosarcoma model and considering our previous studies showing that iso1Au/IL12 and TNF can individually support the therapeutic efficacy of adoptive T-cell therapy in other tumor models [23, 44], the trifunctional iso1Au/TNF + IL12 might exert synergistic effects with other chemo-immunotherapeutic combinations in different tumors, which is an approach that merits future investigation. The gene discussed is TNF; the disease is fibrosarcoma.