To validate the different expressions of genes in “Ferroptosis” between normal liver and fatty liver, four marker genes, gpx4 [35–40], acyl-CoA synthetase long-chain family member 4 (acsl4) [41, 42], transferrin receptor 1 (tfr1) [43], and Glutamate-cysteine ligase (gcl) [44], were selected for qPCR analysis. Here, ACSL4 is linked to fatty liver disease.