Secondary objectives were to estimate a) the incidence of virologically (PCR or non-structural protein 1 [NS1]) confirmed and probable symptomatic dengue cases (by detection of IgG/IgM antibodies to dengue virus by ELISA or rapid immunochromatographic test) by age, gender, serotype (if applicable), and b) the prevalence of anti-DENV IgG antibodies at enrolment, overall and by age, as well as c) to describe the clinical presentations of dengue cases. This evidence concerns the gene CD40LG and dengue disease.