To further explore TWIST1’s potential in driving medulloblastoma cell migration, we inhibited its expression in the highly expressing TWIST1 metastatic cell lines D283Med (M2) and ONS-76 (Supplementary Figure S4A) either genetically using shRNA or chemically using Harmine.36 shRNA treatment resulted in a significant reduction in TWIST1 gene expression in the D283Med and ONS-76 cell lines by 63% and 68%, respectively, and a comparable reduction in protein expression (Supplementary Figure 4B and C). This evidence concerns the gene TWIST1 and medulloblastoma.