Using microarray platforms and high-throughput functional proteomic profiling, genes with mRNA expression levels either positively or negatively correlated with at least one of four EMT markers (i.e., E-cadherin, vimentin, N-cadherin and/or fibronectin 1) and bimodal distribution patterns were used to generate a 76-gene EMT signature indicating whether a NSCLC cell line had undergone EMT. The gene discussed is VIM; the disease is non-small cell lung carcinoma.