ZC3HAV1 and cytomegalovirus infection: In order to understand the effect of ZAP on the viral gene expression cascade over time, we analyzed the whole-transcriptome landscape during HCMV infection by RNA-sequencing (RNA-seq) in WT, control cells, and two independent ZAP KO cell lines (g1 and g3 ZAP KO) (Fig. 5A, Data set S2).