To decipher whether ZAP affects HCMV mRNA expression, we analyzed mRNA levels of the early UL44 and the late UL83 transcripts at different stages of the HCMV infection cycle by quantitative reverse transcription-PCR (qRT-PCR) in WT and ZAP KO cells. The gene discussed is ZC3HAV1; the disease is cytomegalovirus infection.