The E3 ligase tripartite motif protein 25 (TRIM25)-mediated attachment of Lys63-linked ubiquitin chains activates the viral sensor, a retinoic acid-inducible gene I (RIG-I), resulting in the stimulation of the production of the cytokines IFN-α and IFN-β upon viral infection. This evidence concerns the gene TRIM25 and viral infectious disease.