Thus, for the sake of avoiding any possible off-target effects and facilitating meaningful biological comparisons among cellular samples of all different Sos genotypes, in this report we routinely used this procedure (GFP-Adeno-Cre infection) in all experiments involving Sos1 depletion, and all counterpart cellular samples of the other two genotypes (WT and Sos2 KO) were also treated under the same conditions. Here, SOS2 is linked to infection.