NCKAP1 and infection: L. japonicus mutants 121F-specific p53 inducible RNA (Ljpir1) and nck-associated protein 1 (Ljnap1) [76], as well as the M. truncatula required for infection thread (Mtrit–1) mutant (ortholog Ljnap1) [117] were characterized by a similar phenotype (disorganization of the actin cytoskeleton, no reorganization of F–actin in response to inoculation, a decrease in the number of microcolonies in curled root hairs) [76,116].