CD8A and hepatocellular carcinoma: We found an enrichment of CD4+/FOXP3+ over total CD4+ T-cells in IT (52.7%) over PT regions (13.0%, p = 0.004) in primary but not in secondary samples (46.9 versus 66.2%, p = 0.18), whereas the proportion of CD8+/PD-1+ cells was similar in IT and PT regions in both primary (48.3% versus 21.7%, p = 0.08) and secondary HCC (24.8% versus 23.8%, p = 0.92) (Figure 1D).