After in vitro immunofluorescence confirmed specificity of the CD105-targeted microbubbles to CD105 positive endothelial cells (MS1 cell line) compared to 4T1 breast cancer cells, adequate positive correlation in the glioblastoma model between ex vivo CD105 immunofluorescence and in vivo ultrasound dTE (ρ = 0.86) was shown. This evidence concerns the gene ENG and glioblastoma.