Treatment with propranolol also reduced an IL-33-mediated increase in the number of MCF-7 cell colonies in the soft agar assay (Figure 5E,F), indicating that LPIN1 induced by the COT/JNK1/2 pathway is essential for anchorage-independent proliferation of breast cancer under IL-33 stimulation. This evidence concerns the gene LPIN1 and breast carcinoma.