This was demonstrated by anticancer treatment using genetically engineered HEK293T cell-derived EVs containing inactive cytosine deaminase fused with uracil phosphoribosyl transferase (CD-UPRT) administered to glioblastoma cells cultured in 2D monolayers, 3D spheroids and a xenograft glioblastoma mouse model [108]. This evidence concerns the gene UPRT and glioblastoma.