In addition, EVs derived from CSC-rich colonospheres were found to contain 5′-triphosphate RNA that activates NF-kB in neutrophils and increase interleukin-1 beta (IL-1β) secretion, leading to prolonged neutrophil survival as well as promoted tumor cell survival in the primary TME and peritoneal spreading of tumor cells in vivo. This evidence concerns the gene IL1B and neoplasm.