NOTCH3 and Alzheimer disease: The possible reasons are as follows: First, the relatively small sample size in our research (only 365 controls) may lead to a negative result in association analysis, particularly for rare variants; second, the inclusion and exclusion criteria varied among different studies; third, racial differences may account for the inconsistencies in the association analysis between NOTCH3 gene variants and AD; and finally, our targeted capture sequencing mainly focused on variants in the coding regions and adjacent intron regions of NOTCH3, while most known AD risk loci are in non‐coding regions.