In the current study, we detected multiple enriched proteins in the AD pathways, including mitochondrial dysregulated proteins (PHB, SLC25A5, UQCRH, MPPB), Ca2+ flow imbalance (ITPR1, ITPR2), non‐amyloid protein production related proteins ADAM10, endoplasmic reticulum protein RTN4. This evidence concerns the gene PHB1 and Alzheimer disease.