The sample collection in combination with clinical chemistry analysis of 30 parameters as described in the wellness study, anthropometric measurements as well as analysis of insulin, C-peptide, and the homeostatic model assessment of insulin resistance (HOMA-IR) were conducted at the start of the trial (visit 1) and after approximately 1 month (visit 2) and 3 months (visit 3) of treatment (Fig. 1h) (Supplementary Data 2). This evidence concerns the gene INS and Insulin resistance.