We performed the genetic characterization of agnospheres and the corresponding available human tissues (hereafter indicated as “original tissues”), by whole-exome sequencing (Supplementary Table 3): AS901 and AS67 resulted hypermutated, consistently with the presence of a POLE mutation in AS901, and POLE and POLQ mutations in AS67; AS906, AS43, and AS914 harbored different combinations of cancer-associated genes. The gene discussed is POLE; the disease is cancer.