The invasion and migration ability of CML cells were tested by Transwell assay, and the results demonstrated that in K562 cells, the number of invasive and migratory cells was decreased in the sh-HOTAIR group and the si-DNMT1 group relative to that in the sh-NC group and the si-NC group (all P < 0.05) (Fig. 4A, B). Here, DNMT1 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.