Our findings, after adjusting for non-specific binding by subtracting absorbance from uncoated wells, are opposed to those of Taikashi et al., who reported higher levels in patients with Crohn’s disease.50 Whether the fine specificity of anti-HMGB1 Abs that occurs in a disease state implies that these Abs have functional activity as opposed to what is seen in healthy states remains to be clarified. Here, HMGB1 is linked to Crohn disease.