In vitro migration and invasion assays were used to detect the metastatic ability of these A375 cells, and the mGPDH‐ko shRNA‐transduced A375 cells showed greater metastatic ability than the control shRNA‐transduced cells, while the mGPDH‐NRF2‐double ko group displayed numbers of migrated cells similar to those of the control group (Figure 4B,C), indicating that the loss of mGPDH accompanied by increased NRF2 expression contributed to A375 cell migration and invasion, while a genetic reduction in NRF2 levels rescued the melanoma phenotype to a relative normal phenotype. This evidence concerns the gene KRT8 and melanoma.