RT-PCR: Increased IFN-b and IFN-a mRNA in circulating PBMC. ELISA: Increased serum IL-12 and IL-1b concentrations, especially in patients presenting stable disease. IFN-γ- ELISpot: IFNγ-ELISPOT reactivity to tumor lysates was observed in two patients experiencing durable stable disease. This evidence concerns the gene IL1B and neoplasm.