IFN-γ ELISpot: CTLs specific for both VEGFR1 and 2 were induced after vaccination in 2/4 patients. Multiplex IF: In the post-vaccination tumor, expression of cleaved caspase 3 was co-localized in endothelial cells with CD34- and Foxp3-positive cells. No changes in the number of CD163, CD8, and CD4-positive cells after vaccination. Here, CD34 is linked to neoplasm.