Delayed-type hypersensitivity: Skin reaction was observed in nine of ten patients (90.0%) after vaccination. Tetramer: WT1-specific CTLs were detected after vaccination in 4/5 patients. IHC: The immunohistochemical analysis suggested a possible relationship between induction of WT1-specific cytotoxic T lymphocyte after DC vaccination and higher infiltration of CD3/CD4/CD8 lymphocytes in tumor tissues. The gene discussed is CD4; the disease is neoplasm.