WT1 HLA-tetramer assay: A significant increase in the positivity of WT1-specific CD8+ T cells was observed in SD patients after DC vaccination. IFN-γ ELISpot: Marked augmentation of WT1-specific response by DC vaccination was observed Flow cytometry: WT1 specific immunity and the reduction of regulatory T cells. The gene discussed is CD8A; the disease is Salla disease.