The results of immunohistochemical staining indicated that diffuse EAAC1 transporter staining was shown in Hep3B hepatoma (Figure 6C), while minimal EAAC1 staining was observed in normal hepatic tissue (Figure 6D), which suggested that the transport of [18F]AlF-NOTA-NSC-GLU in the Hep3B cell line was likely to involve glutamate transporter EAAC1. This evidence concerns the gene SLC1A1 and hepatocellular carcinoma.