Note that anti-CTLA-4 and BEMPEG treatments were investigated separately and in combination in a preliminary experiment and did not appear to have a significant impact on tumor growth (Supplemental Figure 3A); however, the treatment combination of anti-CTLA-4 and BEMPEG did result in less metastatic disease (Supplemental Figures 3B-C). This evidence concerns the gene CTLA4 and metastatic neoplasm.